A physiological study of the interaction effect of acetate and phosphorous on the growth of some chemical characteristics of (Ocimum basilicum vara viride) and its effectiveness against pathogenic bacteria

Abstract

In this study, two experiments were conducted, one in a field and one in a laboratory. The field experiment took place on 10/1/2023 in a private farm in the agricultural village of Sayed Hussein in the Al-Rifai district, Dhi Qar Governorate, using a plastic canopy. The laboratory experiment involved studying chemical compounds in Al-Fadil Foundation for Training and Development in Babylon Governorate and bacterial compounds in Al-Rifai General Hospital. The study examined the effects of different levels of potassium acetate (CH3COOK) and phosphorous (NPK High phosphorous) on the content of flavonoids, tannins, and phenols in leaves, as well as the inhibitory activity of basil extract against two types of pathogenic bacteria: Streptococcus pneumonia (Gram-positive) and Salmonella paratyphi (Gram-negative). Results showed that acetate spraying at level A3 significantly increased the content of flavonoids, tannins, and phenols in leaves, and inhibited both positive and negative bacteria. Similarly, higher levels of phosphorous (P3) led to increased content of these compounds in leaves and significant inhibition of the pathogenic bacteria. Furthermore, the interaction between acetate and phosphorous levels had a significant effect on the characteristics of the leaves, with the highest effect observed at level A3P3. This combination also showed significant inhibition of pathogenic bacteria. Overall, the study demonstrated the positive effects of acetate and phosphorous levels on the content of beneficial compounds in leaves and their inhibitory activity against pathogenic bacteria.