The Antiangiogenic Effect of Eriodictyol (C15H12O6) in Ex Vivo and in Vivo Animal Study

Abstract

Background: The Angiogenesis is defined as a process of vascularization from preexistent blood vessels, in particular capillaries. Its critical in many physiological and pathological activities and controlled by a complex balance between anti- and pro-antigenic factors. Eriodictyol is a flavonoid that is part of subclasses of flavanones and is found greatly in citrus fruit, vegetable, and medicinally essential herbs. Objective: This study was aimed at the investigation of the effect of Eriodictyol upon angiogenesis process. Material and Method: twelve- to fourteen-week-OLD male albinorats are used in this study. Eriodictyolwas dissolved in DMSO and then it was seriallydiluted into six concentrations. The rat-aortic-ring assay was mainly used to explore the antiangiogenic effect of Eriodictyol exvivo. The use of Chorioallantoic membrane (CAM) assay in vivo is to measure the inhibition zone of the small capillaries by Eriodictyol. The zone of inhibition was calculated as mean inhibition zone of a blood vessels in mm ± standard deviation. Regarding MTT assay, it was used for the assessment of proliferation inhibition of human umbilical vein endothelial cells (HUVEC) cell line. The data obtained were analyzed statistically. Results: results revealed that Eriodictyol has significant dose dependent inhibiting effect on grow of blood vessels by 96.70% ± 3.5 with the concentration 200μg/ milliliter compared with -ve control. It also showed that Eriodictyol is a toxic antiangiogenic compound through the MTT assay through the logarithmic and linear regression equations with a value of 5.34. Conclusion: findings suggested that the activity of Eriodictyol can hugely diminish blood vessel growth in the assay of rat aorta rings, CAM and MTT. Eriodictyol has a repressive activity on tumorgenesis which could take an advantage like an anti-angiogenic antiprolifrative compound.